Gene Cloning - 1 जीन क्लोनिंग - 1

Partner: Udemy
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Description: Way back in 1975 the term "Gene Cloning" was coined in Geneva. The necessity was to circumvent the enormous complexity of total genomic DNA which was difficult to handle. Thus, this DNA was cut into large number of fragments using some DNA cutting enzymes. The fragments were then fused with self-replicating plasmid and introduced in to the host bacterium. Almost parallel it was realized that DNA from unrelated bacterial species can be also propagated by using the same approach. Thus, inter-specific recombined DNA was rendered functional in a foreign host. The research approach was widened in cloning of animal genes into bacterial host, though resulting in controversies. Despite this, the first commercial application of using gene cloning was patented based on the facts that the experiments provide insight into workings of DNA and cells in health and medicine. Over the time many new technologies developed around cloned DNA regarding its function and potential application in medicine and industries. The fundamental processes of gene cloning have not much changed except that they advanced by incorporating technological inputs.In the present course on Gene Cloning - 1, I explained as to how the complete procedure of cloning of a gene can takes place and how the cloned gene can be further manipulated and applied for advanced experiments. During formulating the course, I came across some challenges in typical gene cloning and included a Lecture on "Positional Cloning" a deviation from main theme. Further, what can be initially done with cloned DNA is to know the product that is protein. Hence, I added a protocol on Agarose Gel Electrophoresis of DNA. A complete procedure starting from preparation of agarose gel, preparation of DNA samples, loading of samples and running the gel and finally visualizing the DNA bands is methodically described. Another protocol on isolation of plasmid DNA from bacteria is given to make you well versed with a technique that repeatedly works. How purity of DNA is checked and ascer
Category: Teaching & Academics > Science > Biotechnology
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Price: 99.99
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Source: Impact
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