Techniques in Biotechnology, Molecular Biology& Biochemistry

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Description: Welcome to "Techniques in Biotechnology & Molecular Biology," a comprehensive course designed to equip you with essential skills in Biotechnology, Molecular Biology and Biochemistry. Whether you're a student, researcher, or industry professional, this course will guide you through six key techniques crucial in the field of Biotechnology & Molecular Biology.Two-dimensional Gel Electrophoresis (2D-GE): Two-dimensional gel electrophoresis (2D-GE) is a powerful laboratory technique used to separate complex mixtures of proteins. It combines two separation methods:Isoelectric Focusing (IEF): Proteins are first separated based on their isoelectric point (pI), which is the pH at which a protein has no net charge.SDS-PAGE: In the second dimension, proteins are further separated according to their molecular weight by applying an electric field through a polyacrylamide gel that contains sodium dodecyl sulfate (SDS).The result is a gel with a two-dimensional pattern of spots, where each spot represents a distinct protein. This technique is widely used in proteomics to analyze protein expression, post-translational modifications, and identify biomarkers.Affinity Chromatography: Affinity chromatography is a technique used to separate and purify specific molecules from a mixture based on their selective binding to a ligand. In this method, a column is packed with a solid matrix (often agarose or sepharose) that is covalently attached to a specific ligand, such as an antibody, enzyme, or small molecule. When a sample mixture is passed through the column, molecules that have a specific affinity for the ligand will bind to the column, while others are washed away. The bound molecules can then be eluted by changing conditions (e.g., pH or salt concentration) to disrupt the interaction between the ligand and the target molecule. This technique
Category: Teaching & Academics > Science > Biotechnology
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Price: 44.99
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